En effet, le solide de l'invention prsente un micro-environnement interne hydrophobe/hydrophile favorable, notamment pour l'incorporation de molcules amphiphiles comme le busulfan.Thus, the solid according to the invention has the advantage of having an unexpected load capacity, never reached in the prior art, especially in the case of Busulfan
In addition, cell viability assay (Trypan blue staining), apoptosis assay (annexin V), histological analysis (Hematoxylin and Eosin staining), confocal microscopy [for immunofluorescence detection of zona occludin 1 (ZO1) alternatively known as tight junction protein 1 (TJP1), SP-C, LysoTracker Green DND-25, epithelial sodium channel (ENaC)] and transmission electron microscopy (as described previously 28 ) was performed to characterize the alv-ALI model
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