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PCR product was extracted from 2% agarose gels, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, United States) according to manufacturers instructions, and quantified using Quantus Fluorometer (Promega, United States)

The first movie was sparked by the actual experience of a Hollywood producer who blacked out at his Vegas bachelor party and woke up disoriented in a strip club

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