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89 There have been minimal studies on the acute impact of ENDS products in individuals with established cardiovascular disease who may be more at risk to negative health effects of ENDS

After exposure, cells were fixed with 4% paraformaldehyde for 10 min, then stained with 1x Phalloidin-iFluor 594 ab176757 (Abcam, Cambridge, United Kingdom) for 1 h, following the assay procedure recommended by the manufacturer

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Furthermore, Big Tobacco has failed to demonstrate credible evidence for their claims that widespread use and marketing of nicotine vaping products has been effective at reducing traditional smoking, reducing public health effects of widespread smoking, or curbing the uptake of smoking among young people