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Generally, heavier individuals may take longer to clear nicotine and cotinine

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The pBDMs cell suspension was centrifuged, and the cell pellet was resuspended in fresh triple-culture medium (2 parts of AX E2 Alveolar Barrier Medium: 1part of RPMI) and added on the apical side at a ratio of 1:7 (pBDMs: AX iAECs)