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This may be because the protein was upregulated in tobacco leaves and downregulated in roots, which leads to increased metabolism of tyrosine, glycine, serine, threonine, and phenylalanine in tobacco leaves but decreases in the roots, accumulating enough IAA synthetic substrate in roots, resulting in an increase of IAA content in the root system after topping

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In nicotine blood testing, two kinds of testing are used: a simpler one that indicates whether the test is positive or negative and a complex one that indicates the degree/level/amount of nicotine and cotinine present

Thanks to the in-built glue, even beginners can roll a cigarette quickly