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Vascular density was presented as a percentage of the particular area occupied by vessels in the en face projection and was automatically calculated by the software

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Abbreviations ALI: Airliquid interface BALF: Bronchoalveolar lavage fluid BK: Large conductance, Ca 2+ -activated, and voltage-dependent K + CBF: Ciliary beat frequency CFTR: Cystic fibrosis transmembrane conductance regulator COPD: Chronic obstructive pulmonary disease e-cig: E-cigarette FOXJ1: Forkhead box protein J1 HBEC: Human bronchial epithelial cells IL-6: Interleukin-6 IL-8: Interleukin-8 KRT5: Keratin 5 MMP-9: Matrix metalloproteinase-9 MUC5AC: Mucin 5AC MUC5B: Mucin 5B PG: Propylene glycol SCGB1A1: Secretoglobin family 1A member 1 TGFB1: Transforming growth factor-beta1 VG: Vegetable glycerin References Jerzynski, T., Stimson, G