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Furthermore, biological variables and pharmacological properties introduce additional complexity: Sampling Intensity: Accurate detection of pulse frequency requires high-frequency sampling (e.g., 5-minute intervals), as less-intensive methods can miss more than 50% of secretory events

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The ROS induced peroxidation and mostly with damage by free radicals like OH are most sensitive to loss of DNA by depurination

Ethics declarations Conflict of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper