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It's a naturally occurring copper-binding peptide that directly stimulates collagen I, collagen III, and elastin synthesis the structural proteins that make up the annulus fibrosus

Liu, T., Liu, Y., Zhao, X., Zhang, L., Wang, W., Bai, D., Liao, Y., Wang, Z., Wang, M., & Zhang, J

TB-500 / Thymosin Beta-4: The Actin Regulator With a Wound-Healing Legacy Thymosin Beta-4 is a 43-amino-acid peptide and one of the most abundant intracellular proteins in mammalian cells, playing a critical role in regulating actin polymerizationthe molecular machinery driving cell movement, division, and tissue repair