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any cloudiness may indicate protein aggregation or microbial contamination Visible particulate matter: Floating particles, fibres, or sediment are a clear contraindication to use Discolouration: A yellow, brown, or otherwise abnormal colour change suggests chemical degradation Persistent foaming: Foam that does not resolve after gentle swirling can indicate denaturation of the peptide Leaking or compromised vial: Any sign of vial damage should result in immediate disposal It is important to note that a solution can appear visually normal yet still have reduced potency due to chemical degradation that does not alter its appearance

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