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Initially approved for type 2 diabetes, they are now also FDA-approved for weight management, cardiovascular risk reduction, obstructive sleep apnea, and metabolic-associated liver disease (steatohepatitis)

The anti-VP2, anti-VP3, and anti-VP1 polyclonal antibodies were generated in-house by immunization of rabbits with recombinant PV1 VP2, VP3, or VP1 protein produced from Escherichia coli , as previously reported 41 , respectively

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The mixture was centrifuged at 10,000 g for 3 min