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Non-hydrolyzable ether backbone for sustained signaling assays PKC- modulation and MAPK/PI3K pathway research context Model membrane biophysics and phase behavior studies Generic substitution of (Rac)-1,2-Dihexadecylglycerol with a simple ester-linked diacylglycerol (e.g., 1,2-dipalmitoyl-sn-glycerol) fails because the ether linkage confers fundamentally different biological and biophysical properties