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Additional constraints include the potential for compounds with redox potentials higher than the Fe 3+ /Fe 2+ couple to overestimate values, and interference from sample chromophores absorbing at 593 nm (e.g., biliverdin), causing overestimation

Concurrently, the emergence of molecularly intelligent photosensitizers that change their photophysical or chemical behavior in response to lesion-specific cues (pH, enzymes, redox) exemplifies how analyte-sensing functionality can be built directly into imaging-therapeutic agents