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The total reaction volume was 25 l, containing: 12.5 l Power SYBR Green PCR Master Mix, 0.5 l of each bacterial primer (including the forward and reverse), 6.5 l of nuclease-free water, and 5 l of DNA

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Large intestinal microbiome: secondary fermentation and metabolic function The microbial composition of the cecum, colon, and rectum is generally similar