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Microdose semaglutide may also be useful for patients who want to test tolerability before moving into a standard-dose pathway

Protein synthesis Proteins were synthesized using automated microwave synthesis conditions on a CEM Liberty PRIME 2.0 system at 0.10 mmol scale using the one-pot coupling/deprotection methodology 20,21

Wegovy (semaglutide) tablets, for oral use" (PDF)

Materials and methods Plasmids, cloning and expression Genes encoding pro-peptides and semaglutide main chain P29 with 4 repeats were cloned and inserted into the pET28a (+) vector as pro-peptides fused at the N-terminus of semaglutide main chain P29, and the recombinant plasmid pET28a-ProPT-SEMP29 was constructed in E