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Temperature control, sterile technique, and injection angle aren't suggestionsthey're the difference between a functional research compound and an expensive vial of denatured protein

The beads were washed with wash buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 2 mM MgCl 2 , 0.5 mM TCEP)

The GI tract provides a starting point for this review focused on the known and proposed mechanisms by which the nutritional status impacts host defense against parasitic nematodes and by which worm infection impacts host nutritional status and metabolism