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Transfected cells were also treated with estradiol for an additional 72 h and apoptotic cells were measured using annexin V staining

Some researchers claim that deficiencies, especially in vitamin D, magnesium, zinc, and B vitamins, directly hinder insulin receptor signaling and ovarian hormone production, contributing to PCOS development

Oral medications like methotrexate, cyclosporine, and acitretin may be prescribed for more severe cases

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