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Unlike many other antioxidants that you must get primarily from your diet, your liver is the main factory for glutathione production
The samples were cultured in pancreatic organoid feeding medium, which consisted of advanced DMEM/F12 supplemented with 10 mM HEPES (Thermo Fisher Scientific, 15630056), 1 GlutaMAX (Thermo Fisher Scientific, 35050087), penicillinstreptomycin, 100 ng ml 1 animal-free recombinant human FGF10 (Thermo Fisher Scientific, 500-P151G-50UG), 50 ng ml 1 animal-free recombinant human EGF (Thermo Fisher Scientific, AF-100-15-1MG), 100 ng ml 1 recombinant human noggin (Bio-Techne, 6057-NG), WNT3A-conditioned medium (30% v/v), RSPO1-conditioned medium (10% v/v), 10 nM human gastrin 1 (Sigma-Aldrich, SCP0152), 10 mM nicotinamide (Sigma-Aldrich, N0636), 1.25 mM N -acetylcysteine (Sigma-Aldrich, A9165), 1 B27 (Thermo Fisher Scientific, 17504001), 500 nM A83-01 (Tocris, 2939/10) and 10.5 M Y27632 (Tocris, 1254/1)
doi: 10.1074/jbc.M116.770974 111 SantosMde SousaM
