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Abbreviations PDCOs: Patient-derived cancer organoids GI: Gastrointestinal NGS: Next-generation sequencing PDOs: Patient-derived organoids 3D: Three-dimensional 2D: Two-dimensional PD-1: Programmed death-1 PD-L1: Programmed death-ligand 1 CTLA-4: Cytotoxic T-lymphocyte-associated protein 4 ALI: Airliquid interface PDXs: Patient-derived xenografts GEMMs: Genetically engineered mouse models AI: Artificial intelligence HCC: Hepatocellular carcinoma CCA: Cholangiocarcinoma TME: Tumor microenvironment GC: Gastric cancer 5-FU: 5-Fluorouracil CAFs: Cancer-associated fibroblasts MSI-H: Microsatellite instability-high CRC: Colorectal cancer SHMT: Single-hit multi-target nCRT: Neoadjuvant chemoradiotherapy LARC: Locally advanced rectal cancer AC: Adjuvant chemotherapy TRG: Tumor regression grade DFS: Disease-free survival PFS: Progression-free survival OS: Overall survival PDAC: Pancreatic ductal adenocarcinoma TK1: Thymidine kinase 1 FLC: Fibrolamellar carcinoma ICIs: Immune checkpoint inhibitors MSCs: Mesenchymal stromal cells PBMCs: Peripheral blood mononuclear cells CAR-T: Chimeric antigen receptor T-cell CAR-NK: Chimeric antigen receptor natural killer cell TNF: Tumor necrosis factor BE: Barretts esophagus EAC: Esophageal adenocarcinoma ROS: Reactive oxygen species Fn: Fusobacterium nucleatum TILs: Tumor-infiltrating lymphocytes PDT: Photodynamic therapy NCCN: National Comprehensive Cancer Network PINs: Photothermal immuno-nanocomplexes DILI: Drug-induced liver injury iPSC: Induced pluripotent stem cell AKI: Acute kidney injury I3A: Indole-3-carboxaldehyde SCFAs: Short-chain fatty acids Reference Bray F, Laversanne M, Sung H, Ferlay J, Siegel RL, Soerjomataram I, et al