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Samples were separated using a Raptor HILIC-Si 2.1 100 mm, 2.7 m LC column (Restek) with a constant flow rate of 400 l/min following a flow gradient starting at 5% Mobile Phase B (H 2 O + 5 mM AmFm +0.5% formic acid) then going up to 40% in 5 min, which was followed by re-equilibration at 5% B for 4 min and 30s

It is important to recognise that sleep disturbances can result from multiple factors, including: Changes in blood glucose levels Gastrointestinal discomfort disrupting rest Weight loss and metabolic changes Anxiety or concerns about new medication Underlying sleep disorders unrelated to Ozempic, including obstructive sleep apnoea (OSA), which is common in people with type 2 diabetes If you are experiencing sleep difficulties after starting Ozempic, it is essential to discuss these symptoms with your GP or diabetes specialist nurse

CT, P = 0.024)

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