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In addition, betaine significantly upregulated the protein expression of AMPK (AMP-activated protein kinase), FGF10 (fibroblast growth factor 10), and ATGL (adipose triglyceride lipase) and suppressed hepatic lipid accumulation in mice, suggesting that betaine prevents NAFLD via FGF10/AMPK signaling pathway ( Betaine supplementation was observed to decrease histopathological alterations and serum markers of hepatotoxicity in animal models of acute and chronic non-alcoholic liver injury