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The digested peptides were reconstituted in 2% acetonitrile/0.1% TFA and 5 l of each sample was loaded onto a PepMap 100 3U 75 m 20 mm reverse-phase trap where they were desalted online before being separated on a 100 2U 50-micron 150 mm PepMap EasySpray reverse-phase column 17

Consider Alternative Treatments : If branded Semaglutide is unavailable or unaffordable, explore other FDA-approved options like Liraglutide (Saxenda) or non-pharmacological weight loss strategies, ensuring compliance with medical guidelines

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Although GLP-1 medications have complex dosing schedules, you can consider switching between GLP-1 medications if you experience intolerable side effects with your current medication, do not get significant results, or due to a drug shortage