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Similarly, inhibition of NF-B activation has been shown to alleviate insulin resistance in L6 muscle cells exposed to hydrogen peroxide (HO) [121]

For the NanoLuc expression vector (NanoLuc plasmid), CMV DNA fragment was amplified from the Cas9 plasmid and replaced with the PGK promoter in the pNL1.1.PGK[Nluc_PGK] Vector (Promega) using the In-Fusion HD cloning kit