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The impact of NAC on metabolic changes relative to placebo was investigated by performing a two-factor (NAC versus placebo) time series analysis (changes relative to baseline/visit 1) within individual subjects

PMID 3800996

Procedure Preheat spectrophotometer/microplate reader for 30 minutes, adjust the wavelength to 412 nm, set zero with distilled Preheat Reagent II in 37 (mammal cell) or 25 (other species) water bath for 30 Blank tube determination: take micro glass cuvette, add 20 L of distilled water, 140 L of Reagent II, 40 L of Reagent III in turn, mix well, place for 2 minutes, and measure 412 nm absorbance AB

This could happen due to: Persistent underlying conditions that hinder absorption