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Cell death was shown by the change in cell viability, which was calculated as: (viable cell)% = (optical density (OD) of untreated sample/OD of treated sample) 100

The intracellular domain is characterized by a Toll/interleukin-1 receptor (TIR) homology domain, which is crucial for signal transduction, allowing TLRs to activate downstream signaling cascades and initiate immune responses [178]

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