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Histology and immunohistochemistry Mouse heart samples were cut in the midline of base-apex-axis and fixed in 10% formalin overnight followed by transfer to 70% ethanol and embedding in paraffin and cutting into in 5-m-thick serial sections

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While most published studies use oral formulations rather than injections, they support the underlying principle that addressing energy metabolism and oxidative stress together may be more effective than targeting either alone

A 1% SDS solution was applied to a 1-cm 2 sterile piece of gauze secured to the hair-free back skin with a bio-occlusive dressing (Tegaderm)