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GSH or Ni-NTA bead washing was performed four times with cold PBS or washing buffer (20 mM Tris-Cl (pH 8.0), 300 mM NaCl, 20 mM imidazole)

The catalytic rate constant for superoxide dismutation of a derivative of malonic acid with fullerene C 60 is unusually high (2 10 6 mol 1 s 1 ), similar to the rate constants determined for Mn-based SOD mimics and two orders of magnitude lower than those reported for the SOD enzyme