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DNA dot-blot analysis Genomic DNA from erythroblasts was extracted using phenol:chloroform:isoamyl alcohol (25:24:1) and precipitated with ethanol before dissolving in water

Its sterile formulation and preservative content make it a practical choice for reconstituting lyophilised compounds and maintaining solution integrity when handled appropriately

[3] Purification and identity confirmation After refolding, research-grade preparations are purified by chromatographic methods such as reverse-phase and ion-exchange high-performance liquid chromatography (HPLC)

Results Experimental results Vitamin B 12 and GSH are uncorrelated in type 2 diabetic patients We analyzed vitamin B 12 levels and blood GSH concentrations in Indian diabetic patients and control subjects