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The cells were then washed with PBS three times and incubated with 500 l FITC-labeled DNZ-NLPs and FITC-labeled DNAzyme with a final concentration of 200 g/mL at 37 C for 4 h

We therefore measured G protein-dependent cAMP generation and -arrestin-1 and -2 recruitment with exendin-4, exendin-phe1, and exendin-asp3 at two time-points to avoid kinetic artefacts 38 , and calculated the pathway bias by fitting the response data to a modified form of the operational model of agonism 39 (Fig

this balance influences hemostasis, inflammation modulation, angiogenesis, and tissue remodeling, which are key processes implicated in endometriosis pathogenesis