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Moreno-Jimnez EP, Flor-Garca M, Terreros-Roncal J, Rbano A, Cafini F, Pallas-Bazarra N, et al
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(2017), Acquired Amino Acid Deficiencies: A Focus on Arginine and Glutamine
(3) carrying out gradient elution by taking the reversed-phase silica gel filler as the stationary phase, a saline solution with a pH value adjusted to 2-3 by phosphoric acid as the mobile phase Aand a mixed solution of acetonitrile and isopropanol as the mobile phase B, collecting fractions, and carrying out rotary evaporation to remove a part of the solvents so as to obtain a secondarily-purified solution
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