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Freezing reconstituted peptide damages the molecular structure through ice crystal formation and reduces bioactivity
The high initial GFP intensity levels observed immediately after DEM exposure could be attributed to a delay of approximately 20 min between the addition of DEM and image acquisition (Fig
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It is a concerted process controlled with very regular timing, occurring between 60 s and 120 s from the recruitment of the early adaptor proteins to the membrane and the excision of a vesicle 11
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