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The GSH/GSSG content and ATP levels of 0.51 10 5 cells/well were measured by a luminescent-based assay (Promega V6612 for GSH/GSSG and Promega G7571 or Sigma MAK135 for ATP) following the manufacturers protocol

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Heterodimerization of GHS-R1A with the dopamine receptor D2 has recently been shown to occur in mouse hypothalamic neurons that regulate appetite (Kern et al., 2012) Since GHS-R1a acts as an allosteric modulator of D1 and D2 signaling, this finding implies a functional role for the expressed GHS-R1a in brain areas that may be less accessible to peripherally produced ghrelin and where there is no local production of ghrelin (Jiang et al., 2006

We then measured the effect of BPC 157 on nitric oxide production in vascular endothelial cells and examined the role of nitric oxide in BPC 157-induced cell migration