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After overnight culture, cells were transfected for 24 h with GPCR expression vector, a luciferase reporter vector, and pGL4.74[ hRluc /TK] (Promega) which were preincubated with prepared PEI MAX (Polysciences Inc., Warrington, PA, USA) 52 and Opti-MEM (Thermo Fisher Scientific), followed by incubation with curcumin or its related compounds for 24 h (NFAT reporter) or 4 h (the other reporters)

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