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The column was then washed with 20 CV of DPBS/0.05% (v/v) sodium azide, followed by elution with 9 CV of 2 M MgCl 2 (Sigma-Aldrich)/20 mM Tris-HCl (Sigma-Aldrich) buffer (pH 7.4) at a flow rate of 2 mL/min

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Increased expression of VEGF in the brain has been associated with cerebral ischemia, increased BBB permeability and pathological vascular remodeling, which may contribute to the progression of neurodegenerative diseases

Risks and Side Effects While glutathione IV therapy is generally safe, some people may experience mild side effects