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The organic layers from two extractions were combined and aliquots were analyzed on a 4000 Q-Trap mass spectrometer (AB SCIEX, Framingham, MA) using the 50 2.1 mm Hydrophilic Interaction Liquid Chromatography (HILIC-LC) column (Waters Corp., Milford, MA)

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Diabetes mellitus, which is a complication of obesity, can activate satellite glial cells of the supracervical ganglia through P2Y receptors, resulting in increased expression of pro-inflammatory cytokines such as IL-1, and causing oxidative damage to the autonomic nerves such as via an inflammatory response (111, 112) ( Figure 2 )

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