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The inflammatory response further drives the diseases by increasing oxidative stress, reducing the levels of growth factors, and increasing the release of pro-inflammatory cytokines by activated microglia and astrocytes (Tansey and Goldberg, 2010)

This assay in the presence of hydrogen peroxide (H 2 O 2 ) and horseradish peroxidase (HRP) uses a substituted phenol (3,5-dichloro-2-hydroxybenzenesulfonic acid) that combines with 4-aminoantipyrine to form a red quinoneimine dye

Swelling usually peaks in the first 24 to 48 hours, then eases over 5 to 10 days, with full settling over 2 to 4 weeks

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