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A number of factors influence the type and concentration of glucosinolates found in these vegetables, such as genotype, cultivar, cultivation site, growth conditions (e.g., temperature, nutrient availability, water content), plant stage, plant tissue analyzed, storage conditions, and preparation and cooking methods (Maina et al., 2020)

Nanoparticles were diluted in phenol red-free DMEM/HAMs F-12 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 5% FBS at a final concentration of 100 g/ml and added to the donor compartment (0.2 ml)

Schaeffer AJ (2003) Epidemiology and demographics