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For the OGD phase, cells were washed twice with PBS and then incubated in glucose-free DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) under hypoxic conditions (1% O 2 , 94% N 2 , 5% CO 2 ) in a hypoxia chamber (BioSpherix, Parish, NY, United States) for 8 h
The other part of the hydrolysed GSH molecule cysteinyl-glycine is transported back inside the cell and broken down further by nonspecific dipeptidases into cysteine and glycine , ready for resynthesis back into GSH
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