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( d ) Immunoblot for RIG-I after control or RIGI siRNA mediated knock-down in HepG2-HFL cells and induction of RIG-I expression by transfection of 100 ng in vitro transcribed 5ppp J6/JFH1 RNA, 100 ng Poly I:C or treatment with 500 U/mL IFN-a 2a