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Estrogen and progesterone plummet within 24 to 48 hours of giving birth, creating a hormonal environment that many researchers compare to surgical menopause

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The next day, the membrane was incubated overnight at 4 C with 350 nM human recombinant XPO1 and 1050 nM yeast recombinant GSP1 (RAN1 ortholog) in 2.5% BSA and XPO1 purification buffer