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Comparison of the effects of ICV and iPVN pro-glucagon-derived and related products Comparison of the effects of ICV and iPVN proglucagonderived and related products on food intake in fasted rats

And reacting at room temperature for 3h, adding 10mL of glacial methyl tert-ether, precipitating a white solid, centrifuging, adding methyl tert-ether into the product, washing for 3 times, washing for 2 times with 10mL of ethyl acetate, drying, and purifying by reversed-phase HPLC to obtain 84mg of semaglutide with the purity of 97.52%, wherein the specific liquid-phase chromatogram is shown in FIG

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