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Diagnosis Demographic data were obtained for each subject from medical records at the time of the enrollment and included current age, sex and family history of hypertension and stroke

After incubation with the appropriate antibodies for 30 min, erythrocytes were lysed with lysis buffer (BD FACS Lysing Solution, BD Biosciences, San Jose, USA) for 10 min on a shaker, followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and washing with HBS