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Examination of statistical heterogeneity showed low heterogeneity overall (I 2 = 34%) and no heterogeneity between diagnostic subgroups (I 2 = 0%) (Figure 4)

The NADK gene is located on chromosome 1p36.33 and is composed of 19 exons that generate four alternatively spliced mRNAs that collectively generate three distinct isoforms of the enzyme

Yang Q, Wu F, Wang J et al (2018) Nox4 in renal diseases: an update

The tissues were rinsed with PBS and incubated with Alexa Fluor488 chicken anti-rabbit (1:1000, Life Technologies) for one hour at 37 C and then rinsed with PBS and incubated with Alexa Fluor488 goat anti-chicken antibody (1:1000, Life Technologies) and Alexa Fluor568 goat anti-mouse antibody (1:1000, Life Technologies) for another hour at 37 C