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Phase I and II of the AFWERX SBIR program are the phases funded by the program, in contrast to phase III

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Poor technique, like vigorous shaking, using the wrong solvent, or ignoring sterility, can cause foaming (which risks oxidation), precipitation (insoluble aggregates), or rapid degradation from bacterial byproducts

For normalization of the assay results, the erythrocyte lysate was analyzed for HGB concentration using an automated analyzer (Celltac- MEK 6450, Nihon Kohden, Qinlab Diagnostik, Weichs, Germany)