Cells were seeded at a density of 2.7 10 4 cells/cm 2 in culture flasks and were cultured in Williams Medium E (GIBCO, Darmstadt, Germany) containing 10% (v/v) fetal calf serum (FCS) (Capricorn Scientific, Ebsdorfergrund, Germany), 2 mM L-glutamine (Thermo Fisher Scientific, Darmstadt, Germany), 5 g/mL insulin (Sigma-Aldrich, Steinheim, Germany), 50 M hydrocortisone-hemisuccinate (Sigma-Aldrich) and 1X antibiotic antimycotic solution (AAS) (Sigma-Aldrich)
One participant from the hydroquinone group withdrew from the study due to reasons unrelated to adverse effects
Consider citrate sources : Adding lemon or lime juice to water may help increase urinary citrate, which inhibits stone formation
To prevent these effects caused by fluctuating sugar amounts in the blood, you need to consume meals at consistent intervals and avoid skipping them