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21 DTNB Colorimetric Method Principle: DTNB reacts with the sulfhydryl group of glutathione to produce yellow-colored 5-thio-2-nitrobenzoic acid, which has a maximum absorption peak at a wavelength of 412 nm, while DTNB has almost no absorption peak above 400 nm

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An example is the formation of DON-15-glucuronide (DON-15-GlcA) as the primary human metabolite of DON, which is not observed in most animal species (Maul et al