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The chromatographic column, chromatographic conditions, data preprocessing methods, and statistical analysis procedures were identical to those employed in our previous study [35]
To find the best crystallization conditions crystal screens PACT Premiere HT-96 and Morpheus HT-96 (Molecular Dimensions) were tested mixing 0.2 L of protein with 0.2 L of screen against a 40 L reservoir in MRC 2-lens plates (SWISS-CI) with an Oryx Nano crystallization robot (Douglas instruments)
FUNDING RC is recipient of a fellowship from CAPES
Purity is achieved through an advanced distillation process including fermentation, centrifugation, cell extraction, filtration and crystallization
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