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Materials and Methods GSH/GSSG-Glo Assay (Cat.# V6611) CellTox Green Cytotoxicity Assay (Cat.# G8741) menadione (Sigma, Cat.# M5625) l-Buthionine Sulfoximine (Sigma, Cat.# B2515) digitonin (Sigma, Cat.# D141) HEK-293 cells K-562 cells Note: Cell lines were authenticated using STR genotyping ( GenePrint 10 System, Cat.# B9510)

Unlike many antioxidants that function primarily in either water- or fat-based environments, ALA works in both, allowing it to support antioxidant activity throughout the body.64 Research has also found that ALA may help regenerate other antioxidants, including vitamins C and E