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The tandem mass spectrometer was tuned in the multiple reaction monitoring mode to monitor mass transitions in positive ion mode: CysS-mBB m/z 312 192, CysSS-mBB m/z 344 192, CysSSS-mBB m/z 376 192, CysSSSS-mBB m/z 408 192, GS-mBB m/z 498 225, GSS-mBB m/z 530 192, GSSS-mBB m/z 562 192, GSSSS-mBB m/z 594 192, GSSG m/z 613 355, GSSSG m/z 645 387, GSSSSG m/z 677 339, GSSSSSG m/z 709 371, mBB-S-mBB m/z 432.45 192, mBB-S 2 -mBB m/z 464.55 192, mBB-S 3 -mBB m/z 496.60 192, HS 2 O 3 -mBB m/z 305 225

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Step 8 Cover with a new adhesive plate sealer and incubate at 37C for 60 minutes