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Request the following labs before starting and then at 3 and 6 months: Total testosterone (optimal: 500 to 900 ng/dL for most men) Free testosterone (optimal: 15 to 25 pg/mL) Estradiol (optimal: 20 to 40 pg/mL) SHBG (reference: 10 to 57 nmol/L) LH and FSH (reference: LH 1.8 to 8.6 mIU/mL, FSH 1.5 to 12.4 mIU/mL) If testosterone is rising and estradiol is falling over time, the trajectory is positive regardless of how you feel in the short term

On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

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That number could increase significantly over time as supply shortages of the drug are resolved, as indications for the drugs expand, and especially if health-insurance companies increase coverage for the drugs.