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Free GTP that colocalized in the mitochondria declined in old age Since GTP is primarily produced in mitochondria by the catalytic function of succinyl-CoA synthetase in the TCA cycle, we explored the presence of free GTP pools in the mitochondria in hippocampal neurons, by probing for colocalization with the membrane potential dye TMRM, whose positive charge favors distribution into negatively polarized mitochondria [23]

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Higher-dose oral B12 (e.g., 1000 g daily) is sometimes used but should only be considered under clinical supervision with appropriate monitoring, particularly in cases of malabsorption where intramuscular treatment remains the first-line approach in UK practice

Krizhanovsky, S.W